The AxyPrep MAG Plasmid purification kit utilizes a magnetic beads-based technology for high throughput purification of plasmid DNA from Escherichia coli cells. AxyPrep MAG Plasmid Kit can also be used with fosmid and BAC vector-based constructs ranging from 5 Kb to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralization, DNA binding, ethanol washing, and plasmid elution. The protocol is automation-compatible.