The AxyPrep MAG Tissue-Blood gDNA Kit utilizes a magnetic beads-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instruction to extract gDNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL tubes. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. Genomic DNA is then immobilized on magnetic particles by the addition of a magnetic binding reagent. This differential binding allows the gDNA to be easily separated from contaminants using a magnetic field. Contaminants can then be rinsed away using a simple washing procedure.
- Expanded protocol for animal tissue, cultured cells, buccal swabs, and blood
