Corning® Basic Fibroblast Growth Factors (bFGF), Human Recombinant, 10µg, 1/Pack

(This is the suggested replacement for 356060 and 356061)

Corning® basic Fibroblast Growth Factor (bFGF), human recombinant, 10µg, is a heparin-binding mitogenic protein that enhances the proliferation of a wide variety of cell types under serum-free or serum-reduced conditions. 1/pack, formerly Cat. #40060. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

Lyophilized from phosphate buffered saline, carrier-free; reconstitute in dH2O or serum-free medium

Preparation and Storage

Stable for at least three months at -20°C (lyophilized) or = one month solubilized at -20°C. Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality

• Purity: >95% by SDS-PAGE
• Tested for ability to promote proliferation of FBHEC
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
• Recombinant, E. coli

Molecular Weight

17 kD

Tip

Recommended concentration: 1 to 20 ng/ mL of medium depending on cell type

Storage

• Storage: at least three months at 2-8°C (lyophilized) or one month at -20°C (solubilized)
• Shelf life: >= 3 months from date of shipment