Corning® Interleukin-2 (IL-2), Human Recombinant, 50,000 BRMP Units, 1/Pack

Corning® Interleukin-2 (IL-2), human recombinant, 5000 BRMP units, promotes long-term proliferation of activated T-cells. Also known as T-cell growth factor, Interleukin-2 (IL-2) plays a central role in cell-mediated immune response. 1/pack, formerly Cat. #40043B These cytokines have been used to promote long-term proliferation in cell culture and to induce cell-mediated immune responses in a variety of cell types.

Formulation

Frozen in 55 mM sodium phosphate buffer containing 53 mM sucrose and 0.4% HSA

Preparation and Storage

Stable for at least three months at -70°C; =one month solubilized at -70°C

Tip

Recommended concentration: 10 BRMP units/ mL of medium depending on cell type

Quality

• Purity: >96% by SDS-PAGE
• Tested for ability to stimulate proliferation of the mouse T-cell line CTLL
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
• Tested for endotoxin (LAL assay)

Source

Recombinant, E. coli

Molecular Weight

15.5 kD

References

1. Hatakeyama, M. and Taniguchi, T., Interleukin-2 in Peptide Growth Factors and Their Receptors I, Springer-Verlag, NY (1990)
2. Kashima, N., et al., Nature 313:402 (1985)
3. Leonard, W.J., et al., Proc. Natl. Acad. Sci. USA 80:6957 (1983)
4. Mori, H., et al., Int. Immunol. 3:149 (1991)
5. Morgan, D.A., et al., Science 193:1007 (1976)
6. Smith, K.A., Science 240:1169 (1988)
7. Meidema, F. and Melief, J.M., Immunol. Today 6:258 (1985)
8. Triachiesi, T., et al., J. Exp. Med. 16:1147 (1984)
9. Grimm, E.A., et al., J. Exp. Med. 155:1823 (1982)

Storage

Shelf life: >= 3 months from date of shipment at -70°C or >= one month solubilized at -70°C