Tryptic Soy Broth is a sterile-filtered, ready-to-use solution for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows for the assessment of the capability of an aseptic manufacturing process. Due to the rich nutrient base, TSB may be used in the cultivation of fastidious micro-organisms. This growth medium has animal-free components, making it ideal for testing the sterility of animal-free biopharmaceutical processes.