Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN appears to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.
Applications include:
- Promotion of cell attachment and spreading
- Rapid expansion of cell populations
- Serum-free or reduced-serum culture
- Cell adhesion assays
- Studies of effects of HFN on cell behavior
- Improving survival of primary cells in culture
Source
NOTE: Source material tested for hepatitis B antigen and HIV-1 antibody
Quality Control
- Tested for ability to promote attachment and spreading of BHK-21 hamster kidney cells
- Tested and found negative for bacteria and fungi
- Fibronectin purity >90% by SDS-PAGE
Storage