Excess dye terminator clean-up is an essential step prior to sequencing. The carry-over of excess dye into sequencing results in dye blobs, which leads to overwhelming noise and failed calls of sequenced bases and potentially leading to the failure of the sequencing run. AxyPrep MAG DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean-up of excess dye terminator post-sequencing reaction in Sanger sequencing. The protocol mainly consists of binding, washing and elution steps, and can be performed directly in the thermal cycling plate and requires no centrifugation or filtration.
- Saving time and money: Signifcant BigDye savings without incurring extra cost and processing time with the unneeded BigDye signal enhancer buffer, while maintaining high QV20+ scores
- High signal to noise ratio resulting in long reads and high QV20+ scores
- Suitable for the clean-up of sequencing reaction of different Sanger sequencing-based platforms – ABI® Prism 3730, 3730xl, 3700. 3130, 31030xl, and 3100 – GE Healthcare MegaBACE
- Simple and easy sequencing reaction clean-up process: 25 minutes/96 samples
- No centrifugation or filtration required
